Diversity and Prevalence of Indigenous Soil Bacillus spp. in the Major Cabbage Growing Agroecological Zones of Uganda
Résumé
Different species of genus Bacillus have been reported from different environments of the world. They are reported to play a role of soil fertility improvement, plant growth promotion and disease and pest management. Most of these reports on Bacillus species are from studies conducted outside Uganda and therefore information on the prevalence and diversity of bacillus species in Ugandan soils is scanty. This study aimed at determining the prevalence and diversity of Bacillus spp. isolated from the cabbage rhizosphere in the four major cabbage-growing agroecological zones of Uganda. The experiment was conducted in a laboratory at the College of Veterinary Medicine, Animal Resources and Biosafety Makerere University for morphological and biochemical identification of the Bacillus bacteria. DNA extraction and PCR were conducted at the College of Natural Resources Makerere University while sequencing was done at Macrogen laboratories in Korea and Inqaba Biotec in South Africa. Morphological, biochemical and genomic analyses revealed five Bacillus spp. (22 Bacillus strains) grouped as B. cereus, B. mycoides, B. thuringiensis, B. megaterium and B. bingmayongensis. B. cereus and B. megaterium were the most dominant and widely spread Bacillus spp. A phylogenetic tree indicated three major clads, showing that B. thuringiensis was closely related to B. cereus while B.bingmayongesis was closely related to B.megaterium. The B. mycoides were closely related to some B. cereus strains and B. bingmayongensis. The phylogenetic tree further showed that some Bacillus strains of the same species were distantly related. It was therefore concluded that most abundant and prevalent Bacillus spp. in Ugandan soils were B. cereus and B. megaterium. The presence and abundance of these bacillus species in the Ugandan soil presents an opportunity for soil scientists to innovatively manipulate them for use as biofertilizers and biopesticides for crop production and management. Such innovations would reduce the reliance of farmers on synthetic fertilizers that are pollutants to the environment and unhealthy to the users and consumers
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